• Get approved to download dbGaP data. I did this through eRA Commons.
• Download IBM’s Aspera CLI: download is available here. Because I was downloading to CRI, I selected the Linux x86_64 version.
• Run the downloaded file. You might have to run chmod to make it executable. It should create a directory .aspera/ in your home directory.
"%ASPERA_CONNECT_DIR%\bin\ascp" -QTr -l 300M -k 1 -i "%ASPERA_CONNECT_DIR%\etc\asperaweb_id_dsa.openssh" -W <some_long_key> <some_dbGaP_server_address> .

• Change "%ASPERA_CONNECT_DIR%\bin\ascp" to aspera. If you’re running on CRI, the command is already in the PATH
• Change "%ASPERA_CONNECT_DIR%\etc\asperaweb_id_dsa.openssh" to ~/.aspera/etc/asperaweb_id_dsa.openssh. I’m not entirely sure if specifying a private key is necessary, but this is what I did.
• Run the command in the directory you want the data to go, or edit the . at the end of the command. Wondering where you should put the data? Read all of the section on decrypting, because the data needs to be inside a certain directory to be decrypted.

## Decrypting

I decrypted using NCBI’s SRA Toolkit.

• Because I was working on CRI, I downloaded the Linux version from here.
• The project key (necessary for decryption) can be found on the My Projects tab of the dbGaP data portal. Download the key and move it to CRI.
• To install and configure the SRA Toolkit, I used this guide which was very helpful.
• The guide also walks through how to configure the toolkit, which seems like a lot because I ended up using just one command, vdb-decrypt, but it was necessary.
• When in the interactive program vdb-config -i, you need to do three things: set a home workspace, import the project key (the one you downloaded earlier), and set a project directory for the project corresponding to the key.
• I have the home workspace in my directory inside our CRI lab share, and the project directory inside the home workspace. 71954 and 71955 are two data downloads corresponding with this project.
ncbi
-- dbGaP-11644
|-- files
|   |-- 71954
|   -- 71955
|-- nannot
|-- refseq
|-- sra
-- wgs

• To be decrypted, the data must be inside the project directory that you chose, so in this case, all of the data must be inside dbGaP-11644.
• When the project directory is set up and the data is inside, you can use the vdb-decrypt` command to decrypt the data (SRA data is decrypted differently, and there’s a lot of documentation on the NCBI website about how to deal with that data.)

### Suggest changes

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