ARIC EA hg38 validation height

Author

Sabrina Mi

Published

September 8, 2021

::: {.container-fluid .main-container} ::: {#header .fluid-row} # ARIC EA validation {#aric-ea-validation .title .toc-ignore}

2020-12-22

:::

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::: tab-content ::: {#summary .tab-pane .fade .in .active} Last updated: 2021-09-08

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/Users/sabrinami/Github/ARIC/test_data/GWAS test_data/GWAS
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:::
title: ARIC EA hg38 validation height author: Sabrina Mi date: ‘2021-09-08’

workflowr

Last updated: 2021-09-08

Checks: 5 2

Knit directory: ~/Github/ARIC/

This reproducible R Markdown analysis was created with workflowr (version 1.6.2). The Checks tab describes the reproducibility checks that were applied when the results were created. The Past versions tab lists the development history.

R Markdown file: uncommitted changes

The R Markdown is untracked by Git. To know which version of the R Markdown file created these results, you’ll want to first commit it to the Git repo. If you’re still working on the analysis, you can ignore this warning. When you’re finished, you can run wflow_publish to commit the R Markdown file and build the HTML.

Environment: empty

Great job! The global environment was empty. Objects defined in the global environment can affect the analysis in your R Markdown file in unknown ways. For reproduciblity it’s best to always run the code in an empty environment.

Seed: set.seed(12345)

The command set.seed(12345) was run prior to running the code in the R Markdown file. Setting a seed ensures that any results that rely on randomness, e.g. subsampling or permutations, are reproducible.

Session information: recorded

Great job! Recording the operating system, R version, and package versions is critical for reproducibility.

Cache: none

Nice! There were no cached chunks for this analysis, so you can be confident that you successfully produced the results during this run.

File paths: absolute

Using absolute paths to the files within your workflowr project makes it difficult for you and others to run your code on a different machine. Change the absolute path(s) below to the suggested relative path(s) to make your code more reproducible.

absolute relative
/Users/sabrinami/Github/ARIC/test_data/GWAS test_data/GWAS
/Users/sabrinami/Github/ARIC/results/SPrediXcan results/SPrediXcan
/Users/sabrinami/Github/ARIC/models models
/Users/sabrinami/Github/ARIC/code code

Repository version: No commits yet

Great! You are using Git for version control. Tracking code development and connecting the code version to the results is critical for reproducibility.

Note that you need to be careful to ensure that all relevant files for the analysis have been committed to Git prior to generating the results (you can use wflow_publish or wflow_git_commit). workflowr only checks the R Markdown file, but you know if there are other scripts or data files that it depends on. Below is the status of the Git repository when the results were generated:

Untracked files:
    Untracked:  .DS_Store
    Untracked:  .Rhistory
    Untracked:  ARIC_EA_hg38_validation.Rmd
    Untracked:  ARIC_EA_hg38_validation_height.Rmd
    Untracked:  PWAS/
    Untracked:  code/
    Untracked:  covariances_EA_hg38.Rmd
    Untracked:  figure/
    Untracked:  models/
    Untracked:  results/
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Definitions

conda activate imlabtools
METAXCAN=/Users/sabrinami/Github/MetaXcan/software
DATA=/Users/sabrinami/Github/ARIC/test_data/GWAS
RESULTS=/Users/sabrinami/Github/ARIC/results/SPrediXcan
MODEL=/Users/sabrinami/Github/ARIC/models
suppressPackageStartupMessages(library(tidyverse))
suppressPackageStartupMessages(library(qqman))
suppressPackageStartupMessages(library(data.table))
suppressPackageStartupMessages(library(RSQLite))
suppressPackageStartupMessages(library(UpSetR))
DATA="/Users/sabrinami/Github/ARIC/test_data/GWAS"
RESULTS="/Users/sabrinami/Github/ARIC/results/SPrediXcan"
MODEL="/Users/sabrinami/Github/ARIC/models"
CODE="/Users/sabrinami/Github/ARIC/code"
source(glue::glue("{CODE}/load_data_functions.R"))
source(glue::glue("{CODE}/plotting_utils_functions.R"))

gencode_df = load_gencode_df()

Run S-PrediXcan

Run S-PrediXcan with the ARIC model.

python $METAXCAN/SPrediXcan.py \
--gwas_file  $DATA/imputed_GIANT_HEIGHT.txt.gz \
--snp_column panel_variant_id --effect_allele_column effect_allele --non_effect_allele_column non_effect_allele --zscore_column zscore \
--model_db_path $MODEL/ARIC_EA_hg38.db \
--covariance $MODEL/ARIC_EA_hg38.txt.gz \
--keep_non_rsid --additional_output --model_db_snp_key varID \
--throw \
--output_file $RESULTS/GIANT_HEIGHT_ARIC_hg38.csv

And the mashr model.

python $METAXCAN/SPrediXcan.py \
--gwas_file  $DATA/imputed_GIANT_HEIGHT.txt.gz \
--snp_column panel_variant_id --effect_allele_column effect_allele --non_effect_allele_column non_effect_allele --zscore_column zscore \
--model_db_path $MODEL/mashr_Whole_Blood.db \
--covariance $MODEL/mashr_Whole_Blood.txt.gz \
--keep_non_rsid --additional_output --model_db_snp_key varID \
--throw \
--output_file $RESULTS/GIANT_HEIGHT_mashr_Whole_Blood.csv

Compare Association Results

spredixcan_association_ARIC = load_spredixcan_association(glue::glue("{RESULTS}/GIANT_HEIGHT_ARIC_hg38.csv"), gencode_df)
dim(spredixcan_association_ARIC)
[1] 1318   16
significant_genes_ARIC <- spredixcan_association_ARIC %>% filter(pvalue < 0.05/nrow(spredixcan_association_ARIC)) %>% arrange(pvalue)
spredixcan_association_Whole_Blood = load_spredixcan_association(glue::glue("{RESULTS}/GIANT_HEIGHT_mashr_Whole_Blood.csv"), gencode_df)
dim(spredixcan_association_Whole_Blood)
[1] 12557    16
significant_genes_Whole_Blood <- spredixcan_association_Whole_Blood %>% filter(pvalue < 0.05/nrow(spredixcan_association_Whole_Blood)) %>% arrange(pvalue)

Then compare ARIC and Whole Blood z-scores.

zscores = inner_join(spredixcan_association_Whole_Blood, spredixcan_association_ARIC, by=c("gene"))
dim(zscores)
[1] 814  31
zscores %>% ggplot(aes(zscore.x, zscore.y)) + geom_point() + ggtitle("S-PrediXcan z-score") + xlab("mashr Whole Blood") + ylab("ARIC") + geom_abline(intercept = 0, slope = 1)
Warning: Removed 1 rows containing missing values (geom_point).

We can also look at correlation between z-scores from the ARIC and mashr models.

cor(zscores[c(2,17)], use = "complete.obs", method = "spearman")[1,2]
[1] 0.2915486


Session information

sessionInfo()
R version 4.0.3 (2020-10-10)
Platform: x86_64-apple-darwin17.0 (64-bit)
Running under: macOS Big Sur 10.16

Matrix products: default
BLAS:   /Library/Frameworks/R.framework/Versions/4.0/Resources/lib/libRblas.dylib
LAPACK: /Library/Frameworks/R.framework/Versions/4.0/Resources/lib/libRlapack.dylib

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
 [1] UpSetR_1.4.0      RSQLite_2.2.1     data.table_1.13.2 qqman_0.1.4      
 [5] forcats_0.5.0     stringr_1.4.0     dplyr_1.0.2       purrr_0.3.4      
 [9] readr_1.4.0       tidyr_1.1.2       tibble_3.0.4      ggplot2_3.3.2    
[13] tidyverse_1.3.0  

loaded via a namespace (and not attached):
 [1] Rcpp_1.0.5       lubridate_1.7.9  assertthat_0.2.1 rprojroot_1.3-2 
 [5] digest_0.6.27    plyr_1.8.6       R6_2.4.1         cellranger_1.1.0
 [9] backports_1.1.10 reprex_0.3.0     evaluate_0.14    httr_1.4.2      
[13] highr_0.8        pillar_1.4.6     rlang_0.4.8      readxl_1.3.1    
[17] rstudioapi_0.11  blob_1.2.1       rmarkdown_2.5    labeling_0.4.2  
[21] bit_4.0.4        munsell_0.5.0    broom_0.7.2      compiler_4.0.3  
[25] httpuv_1.5.4     modelr_0.1.8     xfun_0.18        pkgconfig_2.0.3 
[29] htmltools_0.5.0  tidyselect_1.1.0 gridExtra_2.3    workflowr_1.6.2 
[33] fansi_0.4.1      calibrate_1.7.7  crayon_1.3.4     dbplyr_1.4.4    
[37] withr_2.3.0      later_1.1.0.1    MASS_7.3-53      grid_4.0.3      
[41] jsonlite_1.7.1   gtable_0.3.0     lifecycle_0.2.0  DBI_1.1.0       
[45] git2r_0.27.1     magrittr_1.5     scales_1.1.1     cli_2.1.0       
[49] stringi_1.5.3    farver_2.0.3     fs_1.5.0         promises_1.1.1  
[53] xml2_1.3.2       ellipsis_0.3.1   generics_0.0.2   vctrs_0.3.4     
[57] tools_4.0.3      bit64_4.0.5      glue_1.4.2       hms_0.5.3       
[61] yaml_2.2.1       colorspace_1.4-1 rvest_0.3.6      memoise_1.1.0   
[65] knitr_1.30       haven_2.3.1

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